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Chronic administration of bacterial lipopolysaccharide (LPS) produces a suitable model of learning and memory deficits in laboratory rats
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Ismaeil Dalvand   , Samira Choopani , Reza Hamidian , Mohammad Sayyah , Leila Hasanzadeh , Hamid Gholami Pourbadi *  |
| Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran |
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Abstract: (475 Views) |
Background and Aim: Inflammation within the central nervous system indirectly impacts memory and cognition. Bacterial lipopolysaccharide (LPS) serves as a significant contributor to inflammation and is utilized by researchers to simulate and investigate memory impairments such as Alzheimer's disease. Establishing a suitable model is essential for identifying new therapeutic candidates aimed at improving learning and memory. Thus, this study compares two different methods of administering LPS.
Methods: Rats were divided into three groups: sham (PBS), single dose (10 µg LPS), and multiple doses (3 days × 5µg LPS). Intra-hippocampal injections were administered using a stereotaxic apparatus, followed by evaluating the subjects' performance in the Morris Water Maze and shuttle box tests.
Results: The Morris Warer Maze test showed that repeated administration of LPS with a lower dose causes a delay in finding the hidden platform during learning. In the memory test, animals receiving repeated doses of LPS spent less time in the target quadrant than the control or single dose groups (p < 0.05). Also, in the shuttle box test, multiple administration of LPS decreased the delay time in entering the dark chamber and increased the time spent in the dark chamber compared to the control group or single dose (p < 0.05).
Conclusion: The findings suggest that utilizing multiple doses of LPS, as opposed to a single dose, may be a more effective approach in establishing an animal model for studying learning and memory deficits.
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| Keywords: Alzheimer's disease, lipopolysaccharide, hippocampus, learning and memory |
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Full-Text [PDF 2027 kb]
(192 Downloads)
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Type of Study: Original Research |
Subject:
Neuroscience
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