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Investigation of the anticancer effects of inhibiting the transcription factor Sox2 using niosomal nanocarrier containing decoy oligodeoxynucleotides on breast cancer cell line (MDA-MB-231)
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Behrooz Johari   , Vahide Salimi , Hasti Soroushnasab , Saeed Kaboli , Mahmoud Gharbavi *  |
| Nanotechnology Research Center, Research Institute of Basic Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran |
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Abstract: (42 Views) |
key transcription factors such as Sox2 in maintaining pluripotency, the “Differentiation Therapy” approach has been proposed as a targeted and less toxic strategy to disrupt the stemness state of cancer cells. In the present study, the Sox2 transcription factor was targeted and inhibited using decoy oligodeoxynucleotides encapsulated in niosomal nanocarriers, and its anticancer effects were subsequently investigated.
Methods: Niosomes were prepared using the thin-film hydration method and encapsulated with decoy oligodeoxynucleotides for the Sox2 transcription factor. Their physicochemical properties were evaluated using various assays such as FT-IR, DLS and ODNs release. Finally, the blood biocompatibility, cellular uptake rate, and cell viability of the synthesized oligo-containing nanocarriers on a triple negative breast cancer cell line were evaluated.
Results: Physicochemical results showed that the niosomal nanocarrier containing the decoy oligos was successfully synthesized. Hemolysis and cellular uptake studies showed that the synthesized nanocarrier was biocompatible (hemolysis less than 10%) and entered the cells with high efficiency (97.7%), respectively. MTT assay on MDA-MB-231 cancer cells showed that the final formulation (NISM@BSA-DEC) at concentrations of 200 and 300 nM resulted in a significant decrease (p < 0.0001) in cell survival in a dose-dependent manner compared to other treatment groups.
Conclusion: The NISM@BSA-DEC nanosystem can be used as a potential nanocarrier in drug delivery and differentiation therapy for breast cancer. Further cellular and in-vivo experiments are necessary to confirm the efficacy of these nanosystems in future research.
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| Keywords: Decoy oligodeoxynucleotides, Breast cancer, Sox2 transcription factor, Niosomes |
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Type of Study: Original Research |
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