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The effect of aerobic exercise on the prelipin 1 and tail-interacting protein of 47 (TIP47) levels in the liver tissue of obese male Wistar rats
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Seyed Mehdi Sheikhi   , Mehdi Bostani *  |
| Department of Physical Education, Ahv.C., Islamic Azad University, Ahvaz, Iran |
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Abstract: (35 Views) |
Background and Aim: Fat droplets are dynamic organelles that play an important role in body metabolism and energy balance. Therefore, the aim of the present study was to investigate the effect of eight weeks of aerobic exercise followed by twelve weeks of high-fat diet on the levels of prelipin-1 and TIP47 proteins in the liver tissue of male Wistar rats.
Methods: In the present experimental study, 21 male Wistar rats, with eight weeks old and an initial weight of 220 ± 20, were randomly divided into two groups: standard diet (n =7) and high-fat diet (n =14). After inducing obesity for 12 weeks, the rats fed a high-fat diet were randomly divided into two groups: high-fat diet control and high-fat diet aerobic exercise. The aerobic exercise program was performed for eight weeks, five sessions per week, based on the principle of progressive overload. 48 hours after the last training session, the rats were anesthetized with an intraperitoneal injection of a combination of ketamine and xylazine. Then, blood samples were taken directly from the heart, liver tissue was extracted and washed with sodium pentobarbital and stored at -80°C. The levels of perilipin 1 and TIP47 proteins were measured by Western blotting. One-way analysis of variance (ANOVA) with Tukey's post hoc test at a significance level of 0.05 was used for statistical analysis of data using SPSS-23 software.
Results: The results showed that in the high-fat diet group, the levels of perilipin 1 protein in the liver tissue were significantly increased, but there was a significant decrease in the levels of TIP47 protein (p < 0.05). Also, eight weeks of aerobic training resulted in a significant decrease in the levels of perilipin 1 protein and a significant increase in the levels of TIP47 protein in the liver tissue of obese male Wistar rats (p < 0.05).
Conclusion: According to the results of the present study, aerobic exercise by regulating proteins associated with fat droplets can probably be used to adjust energy balance and reduce fat accumulation in obese individuals.
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| Keywords: Physical activity, fat droplets, liver, obesity |
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Full-Text [PDF 645 kb]
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Type of Study: Original Research |
Subject:
Exercise physiology
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