The increasing use of pesticides has raised concerns about their harmful effects on human health. Various evidences indicate a link between pesticide exposure and higher rates of chronic diseases. Mancozeb, a fungicide composed of a polymer of ethylene bis dithiocarbamate (EBDC) with elements of manganese and zinc, has a wide range of agricultural and industrial applications. Recent studies confirm that mancozeb has adverse effects on humans and in different animal models. This effect is due to mancozeb's ability to induce genetic toxicity, mitochondrial dysfunction, and oxidative stress, which are strongly associated with structural changes and functional impairments in organs such as the liver, nervous system, and reproductive system. This article reviews the toxic effects of mancozeb exposure in human and animal models. Also, this compound's main known molecular mechanisms of toxicity are reviewed.