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Effects of acute cuprizone intoxication on inducing depressive-like behaviors in C57BL/6 mice
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Donya Zare   , Ameneh Omidi *  , Mohammad Javan , Forough Foulad , Shahram Pourbeiranvand |
| Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
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Abstract: (59 Views) |
Background and Aim: Multiple sclerosis (MS) is a heterogeneous demyelinating disease of the central nervous system, in which patients experience a wide range of sensory, motor, cognitive, and psychological symptoms. Depression as one of the neuropsychological symptoms is observed with relatively high prevalence in MS patients. This study aims to investigate depression-like behaviors in acute MS model mice induced by a diet containing cuprizone neurotoxin.
Methods: In this study, 14 male C57BL/6 mice were randomly divided into two groups as follows: a group that received a normal diet for 6 weeks (control group) and a group that received a diet containing 2 0.0% received cuprizone poison (cuprizone group). In addition to measuring the weight of the animals, two specific tests related to depression-like behaviors, including the forced swimming test and the tail suspension test, were taken weekly from the mice. At the end of the sixth week, the prefrontal cortex region of the brain tissue underwent histopathological evaluation.
Results: Behavioral evaluations performed by the forced swimming test between the cuprizone and control groups showed a statistically significant difference in the fifth and sixth weeks (p < 0.05). However, the tail suspension test data showed a statistically significant difference between the two cuprizone and control groups only at the end of the sixth week (p < 0.05). Histopathological evaluations showed that following six weeks of a diet containing 0.2% cuprizone toxin, significant demyelination did not occur in the brain's prefrontal cortex.
Conclusion: The diet containing cuprizone neurotoxin can lead to depression-like behaviors in C57BL/6 mice, although the observation of these behaviors was not accompanied by significant demyelination in the prefrontal cortex of the animals.
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| Keywords: Depression, Prefrontal cortex, Cuprizone, Multiple sclerosis, Demyelination
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Full-Text [PDF 887 kb]
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Type of Study: Original Research |
Subject:
Neuroscience
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