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Effect of 8 weeks high-intensity interval training on the stress of liver endoplasmic reticulum in obese rats with type 2 diabetes
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Hadis Bayat   , Mandana Gholami *  , Hamid Rajabi , Hossein Abed Natanzi |
| Department of Professional Physical Education and Sport Science, Kharazmi University, Tehran, Iran |
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Abstract: (527 Views) |
Background and Aim: Recent research has shown that stress of endoplasmic reticulum (ER) in liver tissue plays a significant role in development of type 2 diabetes. The aim of this study was to investigate the effect of 8 weeks high-intensity interval training on two known markers of liver ER stress in rats with type 2 diabetes.
Methods: Twenty four male Wistar rats (8 weeks age and 200-250 g weight) were used. The "healthy control" group (8 mice) had free access to standard food and drinking water for 13 weeks. Another 16 mice were fed a high-fat diet for 4 weeks. Then these 16 rats were made diabetic with streptozotocin and allocated in two groups: "diabetic control" (maintenance of a high-fat diet for 9 weeks without exercise) and "diabetic exercise" (maintenance of a high-fat diet, one week familiarization with treadmill and then 8 weeks high intensity interval training). Then, the expression levels of eukaryotic initiator factor 2-alpha (eIF-2-α) and tumor necrosis factor alpha (TNF-α) in the liver tissue of all rats were measured by western blot method.
Results: Blood glucose level and expression of both eIF2-α and TNF-α were significantly higher in the "diabetic control" than the "healthy control" (P≤0.05). The "diabetic exercise" group showed significantly lower glucose level than the the "diabetic control" group (P≤0.05). However, neither eIF-2-α nor TNF-α showed significant change in "diabetic exercise" compared to the "diabetic control" group.
Conclusion: Intense intermittent exercise with the parameters used in this research could not change two stress markers of liver ER in type 2 diabetic rats.
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| Keywords: endoplasmic reticulum stress, high-intensity interval training, diabetes, obesity |
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Full-Text [PDF 1020 kb]
(201 Downloads)
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Type of Study: Original Research |
Subject:
Exercise physiology
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