[Home ] [Archive]   [ فارسی ]  
Main Menu
Home::
Journal Information::
Archive ::
For Authors::
For Reviewers::
Ethical Code::
Subscription::
Contact us::
Peer Review Process::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 3, Issue 2 (7-2019) ::
Ir J Physiol Pharmacol 2019, 3(2): 147-142 Back to browse issues page
Investigation of the appropriate isolation methods of human dental pulp stem cells from third molars for osteogenic and adipogenic differentiation
Fatemeh Shariatnia , Seyed Adel Moallem , Mahnaz Ahmadimanesh , Elham Ramazani , Zahra Tayarani-Najaran *
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:   (1860 Views)
Background and aims: The aim of this study was to compare different isolation methods to obtain stem cells from adult human third molars pulp and investigation on their potential of osteogenic and adipogenic differentiation.
Methods: Dental pulp was isolated by three ways: 1) direct culture of pulp pieces, 2) enzymatic digestion of pulp using collagenase and dispase, and 3) direct culture of pulp pieces and fixing it with lamella. Stem cell surface markers were analyzed by flow cytometry method. Adypogenic and osteogenic differentiation were verified by Oil Red O staining and Alizarin Red S staining, respectively.
Results: The growth of cells cultured after enzymatic digestion was faster than those isolated by other methods and the use of lamella increased the risk of infection and cell damage.
Conclusion: Dental pulp stem cells isolated with enzymatic digestion has faster growth and might be as an available resource both for research and tissue engineering.
Keywords: Isolation, osteogenic, adipogenic
Full-Text [PDF 1372 kb]   (1232 Downloads)    
Type of Study: Technical Report | Subject: Others...
Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shariatnia F, Moallem S A, Ahmadimanesh M, Ramazani E, Tayarani-Najaran Z. Investigation of the appropriate isolation methods of human dental pulp stem cells from third molars for osteogenic and adipogenic differentiation. Ir J Physiol Pharmacol 2019; 3 (2) :147-142
URL: http://ijpp.phypha.ir/article-1-383-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 3, Issue 2 (7-2019) Back to browse issues page
مجله فیزیولوژی و فارماکولوژی ایران Iranian Journal of Physiology and Pharmacology
Persian site map - English site map - Created in 0.07 seconds with 36 queries by YEKTAWEB 4645