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The role of nitric oxide on ischemic hippocampus during renal ischemic preconditioning
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Fatemeh Zare Mehrjerdi   , Hamidreza Pazoki-Toroudi , Mansoureh Soleimani , Marjan Ajami , Nahid Aboutaleb * |
| 2. Physiology Research Center, Physiology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran |
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Abstract: (13944 Views) |
Introduction: Remote ischemic preconditioning (RIPC) is an episode of intermittent sub lethal and brief ischemia in one organ, which causes protection against severe ischemia in another remote organ. The main purpose of this study was to assess the relevance of nitric oxide (NO) in renal ischemic preconditioning during brain ischemia-reperfusion in hippocampal tissue.
Methods: 60 male BALB/C mice weighting 30-35g randomly were divided into six groups. Sham-operated group, IPC group (transient renal ischemia), IR group (global brain ischemia), RIPC (IPC+IR) group, L-NAME + IPC + IR group (L-NAME was injected 30 min before RIPC), L-NAME + sham + IR group. Three days after brain ischemia, the cell density in hippocampal CA1 subregion and the level of nitric oxide (NO) in hippocampus tissue were measured in different groups.
Results: RIPC prevented the reduction in cell density in hippocampal CA1 area following ischemia (p ≤ 0.05). The level of nitric oxide was significantly increased in the hippocampus in comparison with the IR group (p ≤ 0.05). L-NAME reversed the protective effects of RIPC on ischemic hippocampal damage by inhibition of nitric oxide (p ≤ 0.01).
Conclusion: Increased nitric oxide during RIPC can protect the hippocampus against damage caused by ischemia-reperfusion.
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| Keywords: Brain ischemia-reperfusion, Mice, Nitric oxide, Renal ischemic preconditioning |
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Full-Text [PDF 1087 kb]
(2469 Downloads)
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Type of Study: Original Research |
Subject:
Kidney and Urinary tracts
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