The aim of the study was to investigate the effect of six weeks of TRX training and curcumin consumption on liver enzymes in female athletes. This quasi-experimental study was conducted on 36 female athlete students aged 18-25 years. Targeted subjects were selected and randomly assigned to three groups (12 people). The intervention protocol was: six weeks of resistance training and curcumin supplement consumption (500 mg daily). AST, ALT, and ALP kits were used to measure enzymes. Data were analyzed with paired t-tests and one-way ANOVA at a significance level of p < 0.05. AST values ​​in the exercise+placebo group (p = 0.03) and exercise+curcumin (p = 0.02) decreased significantly, and ALT in the exercise+placebo group (p = 0.01) and exercise+curcumin (p = 0.02) increased significantly, and ALP enzyme in the exercise+placebo group (p = 0.02). Significant differences were observed between the exercise+placebo group and the curcumin group in AST and ALT (p = 0.00) and between the exercise+curcumin group and the curcumin group in AST (p = 0.01) and ALT (p = 0.02). Therefore, the use of curcumin along with TRX training as a non-invasive method will not have a protective role in the health of individuals and cannot have a positive effect on the levels of liver enzymes.