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Changes in the prefrontal cortex glutamate decarboxylase 67 gene expression of immature, adult and old rats in innate anxiety
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Mohammad Javad Esperham   , Sahel Motaghi *  , Mahya Moradi Sirchi |
| Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran |
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Abstract: (845 Views) |
Background and Aim: The age of laboratory animals is an important variable at the time of the experiment. GABA is a neurotransmitter that is synthesized from the amino acid glutamate by the enzyme glutamate decarboxylase (GAD). GAD67 is the main enzyme responsible for the production of GABA and its reduction causes a decrease in GABA levels. Since the prefrontal cortex is one of the important brain structures in the neuronal circuits of anxiety and there has not been a comprehensive study related to anxiety and the change in gene expression of this enzyme in the prefrontal cortex at different ages, in this study, with the help of the elevated plus maze device, this assay was investigated.
Methods: Rats with three age groups of 21, 42 and 360 days were used. At each age, the grouping of animals was as follows: 1) control group, 2) innate anxiety group in which the rats were placed in the elevated maze test, 3) diazepam administration group + innate anxiety group. Immediately after the test, the medial prefrontal cortex of the animals was isolated and evaluated with the help of a real time PCR technique.
Results: Our results showed that anxiety in 21-day-old mice were higher than other ages. GAD67 enzyme expression of the prefrontal cortex did not change in innate anxiety and age had no effect on it (p > 0.05).
Conclusion: It seems that in the prefrontal cortex, other components of the GABAergic system or other neurotransmitters such as serotonin, norepinephrine, etc. and their related mechanisms are involved in innate anxiety.
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| Keywords: Anxiety, Prefrontal cortex, Glutamate decarboxylase 67 |
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Full-Text [PDF 1094 kb]
(248 Downloads)
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Type of Study: Original Research |
Subject:
Neuroscience
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