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Effects of saffron hydro-alcoholic extract on the hippocampal oligodendrocyte transcription factor 2 and glial fibrillary acidic
protein distribution in female demyelinated rats
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Zahra Javid   , Mahnaz Taherianfard *  , Javad Sajedianfard , Hamid Reza Moradi |
| Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran |
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Abstract: (1416 Views) |
Background and aim: Demyelinating diseases are autoimmune disorders of the nervous system. The present study aims to effect of the hydro-alcoholic extract of saffron on blood brain-barrier destruction, Glial fibrillary acidic protein, and Oligodendrocyte transcription factor 2 distribution in astrocyte and oligodendroglia cells and the number of mentioned cells.
Methods: Forty female Sprague Dawley adult rats in four groups, Sham, treatment control, saffron treatment, and infliximab treatment were used. Demyelination was induced by 3 µl of 0.01% ethidium bromide injection in the dentate gyrus. Immunohistochemistry was used, for the distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 determination. The Image Analyzer software have evaluated the distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2. Demyelination was determined by Luxol fast blue staining and image-j software. The ELISA method and Evans blue extravasation measurement have investigated the extent of blood brain-barrier disruption.
Results: Blood brain-barrier disruption was meaningfully (p < 0.05) more in the treatment control compared to the other groups. The distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 and the number of both cells were considerably (p < 0.001) lower in the treatment control relative to the other groups. Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 remarkably (p < 0.05) were lesser in the treatment control compared to the other groups.
Conclusion: The hydro-alcoholic extract of saffron ameliorates blood brain-barrier destruction, improved Glial fibrillary acidic protein, and Oligodendrocyte transcription factor 2 distribution and number of astrocyte and oligodendroglia cells.
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| Keywords: Glial fibrillary acidic protein, Demyelination, Blood Brain Barrier, Oligodendrocyte transcription factor |
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Full-Text [PDF 1241 kb]
(657 Downloads)
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Type of Study: Original Research |
Subject:
Neuroscience
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