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Effect of intracerebroventricular injection of streptozotocin on memory, reactive oxygen species of brain mitochondria, and β-amyloid plaques in rat hippocampus
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Mozhdeh Anjomani   , Afsaneh Eliassi *  , Rasoul Ghasemi , Javad Fahanik-Babaei |
| Department of physiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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Abstract: (1326 Views) |
Background and aims: Alzheimer's disease (AD), is the most common neurodegenerative disease that is associated with cognitive and behavioral disorders and the rapidly increasing prevalence. In addition to its detrimental effects on learning and memory, AD affects the mitochondria as a vital organ of the cell as well as the hippocampal tissue that is the site of learning and memory. In this study, we investigated the concomitant effects of streptozotocin (STZ) on spatial memory, mitochondrial production of reactive oxygen species (ROS), and hippocampal β-amyloid plaques in rats.
Methods: Animals were randomly divided into control, sham and STZ groups receiving streptozotocin 2, 3, 4 and 5 mg/kg through intracerebroventriclular (icv) injection. Learning and memory of rats was examined 14 days later by Morris Water Maze (MWM) test. Moreover, mitochondrial ROS in the brain and β-amyloid plaques in the hippocampus were evaluated.
Results: STZ 3 mg/kg (single dose, icv) was found the effective dose for impairing learning memory in the shortest possible time (14 days). Using Congo red staining, a number of amyloid beta plaques were observed in the hippocampus. Mitochondrial ROS production also increased by this dose of STZ.
Conclusion: Behavioral, biochemical, and histological findings of the present study suggest STZ 3 mg/kg icv injection to rats as one of the acceptable animal models of AD. |
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| Keywords: Alzheimer, Streptozotocin, Memory, Reactive oxygen species, Congo Red, Mitochondria |
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Full-Text [PDF 1267 kb]
(703 Downloads)
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Type of Study: Original Research |
Subject:
Neuroscience
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