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The effect of benfotiamine administration on tensile strength of sciatic nerve and its acellular scaffold in streptozotocin-induced diabetes in rat
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Leila Vafadar-Ghasemi   , Morteza Behnam-Rassouli *  , Maryam Moghadam-Matin , Naser Mahdavi-Shahri |
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Abstract: (3264 Views) |
Background and aims: Neuropathy as a common complication of diabetes might be induced by changes in the extracellular matrix, including the thickening of the basal membrane of the peripheral nerve and elevation of the advanced glycation end products in the extracellular matrix. Benfotiamine inhibits nerve damages induced by hyperglycemia through several pathways. The purpose of this study was to study the effect of Benfotiamine administration on the tensile strength of the sciatic nerve and its acellular scaffold in diabetic rat model.
Methods: Sixty rats were divided into control, sham and 4 and 8 weeks diabetic groups (with and/or without benfotiamine oral treatment; 100 mg /kg). Diabetes was induced by intraperitoneal injection of streptozotocin. At the end of the experimental periods (4 and 8 weeks) 1 cm segment of the middle part of the sciatic nerve was removed, half of it was decellularized by the Sandal method and then subjected to tensile strength test. Thin and semi-thin sections were also prepared for histological examinations.
Results: The mean force for the breaking of nerve segment in the diabetic groups was significantly reduced compared to control group. Also, the length increase up to the breaking point in diabetic groups was significantly shorter than control group. There were no significant differences in the length increase and maximum force in benfotiamine-treated diabetic groups compared to control group
Conclusion: Alteration of extracellular matrix in diabetes condition, might affect the structure of the collagen fibers and therefore reduce the strength of the nerve against the stretch. |
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| Keywords: Benfotiamine, Diabetes, Sciatic nerve, Neuropathy |
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Full-Text [PDF 1138 kb]
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Type of Study: Original Research |
Subject:
Neuroscience
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