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Effect of metformin against hydrogen peroxide-induced apoptosis in human vascular endothelial cells
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Azadeh Aminzadeh *   |
| Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran |
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Abstract: (13372 Views) |
Introduction: Metformin is an anti-diabetic drug, which may be a novel therapeutic option for treatment of atherosclerosis. The current study evaluates protective capacity of metformin in hydrogen peroxide (H2O2)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs).
Methods: Endothelial cells were treated with metformin for 24 h. Then they were treated with 0.5 mM H2O2 for 2h. Cell viability was measured by 3‑(4,5-dimethylthiazol-2-yl)-2, 5‑diphenyltetrazolium bromide (MTT) assay. Reactive oxygen species (ROS) levels were measured using 2, 7 dichlorodihydrofluorescein diacetate (DCF-DA) assay. Lipid peroxidation (LPO), total thiol groups and total antioxidant power (TAP) were also evaluated.
Results: Metformin at concentration of 20 μM increased survival of HUVECs exposed to H2O2. ROS and MDA levels increased in H2O2-treated endothelial cells. Metformin could significantly decrease H2O2-induced ROS and MDA elevation. It also increased total thiol groups and TAP.
Conclusion: Metformin protects HUVECs against the oxidative stress and apoptosis induced by H2O2. |
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| Keywords: Apoptosis, HUVECs, Hydrogen peroxide Metformin |
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Full-Text [PDF 1032 kb]
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Type of Study: Original Research |
Subject:
Cardiovascular System
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