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Biophysical properties of single 346 pS potassium channel of hepatocyte endoplasmic reticulum in diabetic rats
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Maedeh Ghasemi   , Reza Saghiri , Afsaneh Eliassi * |
| Department of Physiology and Neurophysiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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Abstract: (13734 Views) |
Background and aim: Defects in endoplasmic reticulum (ER) homeostasis are common in different diseases, such as diabetes. It is now well established that ion channels of ER membrane have a critical role in ER luminal homeostasis. Our previous studies showed the presence of a 346 pS potassium channel in ER. Therefore, in this study, we examined and compared the activities of this channel in control and diabetic rats using single-channel recording techniques.
Methods: Diabetes mellitus was established in male Wistar rats 2 weeks after single injection of streptozotocin 45 mg/kg, i.p. Ion channel of rough endoplasmic reticulum of diabetic hepatocytes was incorporated into the bilayer lipid membrane and the characterization of K+ channel was then evaluated.
Results: Based on our previous data, endoplasmic reticulum membrane has a potassium channel with a main conductance of 346 pS, which is sensitive to 4-Aminopyridine and voltage-insensitive at ± 30 mV. In the present study, it was demonstrated that the channel conductance was decreased to 259 pS in diabetes mellitus. Interestingly, the channel Po-voltage relation demonstrated that channel Po significantly lower in diabetic rats compared to normal rats at different voltages.
Conclusion: Our data showed that the biophysical properties of potassium channel were significantly altered under diabetic condition. We propose that the hepatocyte ER potassium channels are involved in diabetes pathology, and it may be considered as a target for therapeutic plans.
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| Keywords: Diabetes, Endoplasmic reticulum, Hepatocyte, Potassium channel |
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Full-Text [PDF 1503 kb]
(2317 Downloads)
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Type of Study: Original Research |
Subject:
Electrophysiology
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