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:: Volume 7 - ::
Ir J Physiol Pharmacol 2023, 7 - : 19-31 Back to browse issues page
Effects of saffron hydro-alcoholic extract on the hippocampal oligodendrocyte transcription factor 2 and glial fibrillary acidic protein distribution in female demyelinated rats
Zahra Javid , Mahnaz Taherianfard * , Javad Sajedianfard , Hamid Reza Moradi
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract:   (500 Views)
Background and aim: Demyelinating diseases are autoimmune disorders of the nervous system. The present study aims to effect of the hydro-alcoholic extract of saffron on blood brain-barrier destruction, Glial fibrillary acidic protein, and Oligodendrocyte transcription factor 2 distribution in astrocyte and oligodendroglia cells and the number of mentioned cells. 
Methods: Forty female Sprague Dawley adult rats in four groups, Sham, treatment control, saffron treatment, and infliximab treatment were used. Demyelination was induced by 3 µl of 0.01% ethidium bromide injection in the dentate gyrus. Immunohistochemistry was used, for the distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 determination. The Image Analyzer software have evaluated the distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2. Demyelination was determined by Luxol fast blue staining and image-j software. The ELISA method and Evans blue extravasation measurement have investigated the extent of blood brain-barrier disruption.
Results: Blood brain-barrier disruption was meaningfully (p < 0.05) more in the treatment control compared to the other groups. The distribution of Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 and the number of both cells were considerably (p < 0.001) lower in the treatment control relative to the other groups. Glial fibrillary acidic protein and Oligodendrocyte transcription factor 2 remarkably (p < 0.05) were lesser in the treatment control compared to the other groups. 
Conclusion: The hydro-alcoholic extract of saffron ameliorates blood brain-barrier destruction, improved Glial fibrillary acidic protein, and Oligodendrocyte transcription factor 2 distribution and number of astrocyte and oligodendroglia cells.

 
Keywords: ‎Glial fibrillary acidic protein, Demyelination, Blood Brain Barrier, Oligodendrocyte transcription factor
Full-Text [PDF 1241 kb]   (287 Downloads)    
Type of Study: Original Research | Subject: Neuroscience
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Javid Z, Taherianfard M, Sajedianfard J, Moradi H R. Effects of saffron hydro-alcoholic extract on the hippocampal oligodendrocyte transcription factor 2 and glial fibrillary acidic protein distribution in female demyelinated rats. Ir J Physiol Pharmacol 2023; 7 :19-31
URL: http://ijpp.phypha.ir/article-1-627-en.html


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Volume 7 - Back to browse issues page
مجله فیزیولوژی و فارماکولوژی ایران Iranian Journal of Physiology and Pharmacology
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