|
Sex-related effects on cardiac function, biochemical markers, and oxidative stress in a rat model of heart failure
|
Naser Yousefi   , Sohaila Erfani , Zahra Salimi , Tahere Valadbeigi , Seyedeh Mahdieh Khoshnazar , Nahid Aboutaleb *  |
| Physiology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran |
|
|
Abstract: (18 Views) |
Background and Aim: Advances in cardiovascular research have identified oxidative stress as an important pathophysiological pathway in the creation and development of heart failure. Gender is also associated with differences in oxidative stress. This study aimed to investigate the effect of gender differences on cardiac function, biochemical parameters, and oxidative stress in an isoproterenol-induced heart failure (HF) model in rats.
Methods: Forty Wistar rats (male and female) were divided into four groups: male control, female control, male HF, and female HF. HF was induced by subcutaneous injection of isoproterenol (130 mg/kg) for four consecutive days. On day 28, cardiac function was assessed using echocardiography. Serum levels of CK-MB, LDH, and cTnI were measured to evaluate myocardial injury. Oxidative stress indicators (MDA, SOD, and GSH) were examined using ELISA.
Results: The study revealed no significant sex differences in serum levels of myocardial injury markers (LDH, CK-MB, and cTnI). Ejection fraction (EF) and fractional shortening (FS) were reduced in HF groups, but both parameters were significantly higher in female HF rats than in males (p < 0.05). No significant differences were observed in left ventricular internal diameters. In the female heart failure group, GSH and SOD were higher, and MDA was lower than in the male heart failure group (p < 0.05).
Conclusion: These findings suggest that isoproterenol-induced HF leads to significant alterations in cardiac function, biochemical markers, and myocardial structure, with more severe effects in male rats. The study highlights the importance of considering sex differences in the pathophysiology of HF, which could inform the development of effective therapeutic strategies.
|
|
| Keywords: Oxidative stress, Isoproterenol, Gender, Lactate dehydrogenase, Heart failure |
|
|
Full-Text [PDF 1077 kb]
(7 Downloads)
|
|
Type of Study: Original Research |
Subject:
Reproductive System
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
