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Protective role of genistein against hepatic injury induced by renal ischemia–reperfusion in male rats
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Firouzeh Gholampour *    , Khojasteh Malekmohammad |
| Department of Biology, College of Science, Shiraz University, Shiraz, Iran |
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Abstract: (1 Views) |
Background: Renal ischemia-reperfusion (IR) injury, characterized by increased reactive oxygen species (ROS) generation and depletion of cellular antioxidants, leads to acute kidney injury and impaired liver function. This study aimed to evaluate the protective effects of genistein against liver injury induced by renal ischemia-reperfusion in male rats.
Methods: Twenty-eight adult male Sprague-Dawley rats were divided into four equal groups: control, genistein (15 mg/kg, intraperitoneally, 30 minutes before and 1 hour after ischemia), IR (45 minutes of renal ischemia followed by 24 hours of reperfusion), and IR + genistein. Biochemical parameters including liver enzymes, triglycerides, cholesterol, low- and high-density lipoproteins, albumin, total protein, and bilirubin were measured. Hepatic malondialdehyde (MDA) levels, catalase (CAT) and superoxide dismutase (SOD) activities, and histopathological changes were also evaluated after reperfusion. Data were analyzed using one-way ANOVA and Tukey’s post hoc test.
Results: Renal ischemia-reperfusion (IR) caused a significant (p < 0.001) increase in hepatic tissue damage, liver enzyme levels, plasma lipids, low-density lipoprotein, and bilirubin, accompanied by a significant (p < 0.001) decrease in high-density lipoprotein, albumin, and total protein. Moreover, malondialdehyde (MDA) levels significantly (p < 0.001) increased, while catalase (CAT) and superoxide dismutase (SOD) activities significantly (p < 0.001) decreased. Genistein treatment effectively modulated these alterations, improving liver function, enhancing antioxidant enzyme activities, and alleviating histopathological damage.
Conclusion: Genistein protects the liver against structural and functional damage caused by renal ischemia–reperfusion injury. This protective effect is mediated through attenuation of oxidative stress and enhancement of the antioxidant defense system.
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| Keywords: Acute kidney injury, Oxidative stress, Ischemia-Reperfusion, Genistein, Liver |
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Full-Text [PDF 2073 kb]
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Type of Study: Original Research |
Subject:
Kidney and Urinary tracts
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