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Effects of insulin and somatostatin on water intake in neonatal chickens
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Shiba Yousefvand   , Farshid Hamidi * , Morteza Zendehdel , Abbas Parham |
| Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran |
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Abstract: (6550 Views) |
Background and aim: Disorders of body fluid balance lead to metabolic problems and diseases such as ascites in poultry, which require studies on the clarification of involved mechanisms in water intake. The effect of insulin and somatostatin on water intake in 5-day male broiler chickens (Ross 308) was investigated.
Methods: Two experiments were conducted. In the first one, 10, 20 and 40 ng of insulin and in the second one, 0.5, 1 and 2 nM of somatostatin were injected to chicken via intracerebroventricular (icv) injection. Control group received Evans blue in both experiments. Accumulated water intake at 90 and 180 minutes after injection was measured.
Results: The doses of 10, 20 and 40 (ng) of insulin at 90 min after injection, showed a significant reduction in water intake compared with control group (p < 0.05). In 180 minutes after injection of insulin 20 ng, reduction of water consumption was retained but doses of 10 and 40 ng did not affect water consumption. The group that received 20 ng of insulin showed a significant reduction on water intake 180 minutes after injection compared with groups of 10 and 40 ng (p < 0.05). Somatostatin 0.5nM did not affect accumulated water intake in 90 and 180 minutes after injection compared with control group. However, somatostatin 1 and 2 nM significantly reduced water intake in 90 minutes after injection compared to the control group (p < 0.05). In 180 minutes after injection of somatostatin 2 nM, reduction of water consumption was retained but the dose of 1nM did not affect water consumption. Doses of 0.5, 1 and 2 nM of somatostatin significantly reduced water intake in a dose-dependent manner (p < 0.05).
Conclusion: Somatostatin and insulin reduced water intake in neonatal chickens.
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| Keywords: icv injection, Insulin, Neonatal chickens, Somatostatin, Water intake |
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Full-Text [PDF 1214 kb]
(1106 Downloads)
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Subject:
Endocrine
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