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Extraction of high quality ribonucleic acid molecules from the rat brain by means of an inexpensive method
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Siamak Beheshti *   , Sahar Tohidloo , Azadeh Ghorbanpour-Shakakomi |
| Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran |
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Abstract: (6567 Views) |
Background and aim: Extraction of high quality ribonucleic acid (RNA) molecules from the high fat tissues such as brain has been a great challenge for neuroscience researchers. Expensive kits have been developed to overcome this challenge. However, many researchers are unable to use due to lack of financial resources. In this study a simple and inexpensive method is suggested.
Methods: One month old male Wistar rats were anesthetized by chloroform and their hippocampus was removed. Total RNA of the brain tissue was extracted by RNX-Plus solution from Sinaclon Company. The quality of the extracted RNA was evaluated by Nanodrop spectrophotometer and measuring the 260/280 and 260/230 absorbance ratios.
Results: The mean weight of the dissected hippocampi was 60.2 ± 1.2 mg. The mean absorbance ratio of 260/280 and that of 260/230 were obtained 1.88±0.02 and 1.9±0.03, respectively. The agarose gel electrophoresis indicated presence of the ribosomal RNAs 28s and 18s and the extracted RNA had a good quality. The mean concentration of the extracted total RNA was 608 ng/μl.
Conclusion: The results indicate that it is possible to extract high quality RNA molecules from the brain tissue by an inexpensive method. Therefore, the presented protocol is recommended to molecular neurobiologists. |
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| Keywords: Brain, Extraction, Hippocampus, Rat, Ribonucleic acid |
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Full-Text [PDF 1091 kb]
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Subject:
Neuroscience
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