Effect of aerobic training and aged garlic extract supplementation on TLR4 and NFƙB gene expression in visceral adipose tissue
and lipid profile changes in obese male rats
|
Parisa Khabiri , Mohammad Rahman Rahimi * , Iraj Rashidi , Seyed Ershad Nedaei |
Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran |
|
Abstract: (437 Views) |
Background and Aim: Chronic low-grade inflammation is a common condition in obesity. The aim of the present study was to investigate the effects of aged garlic extract and aerobic training on TLR4/NFƙB inflammatory signaling pathway gene expression in obese male rats.
Methods: Forty male Sprague-Dawley rats were subjected to high-fat diet (n = 32) and standard diet group (n = 8) for 9 weeks. After inducing obesity in the rats fed high-fat diet, the rats of this group were divided into four subgroups (n = 8), including the obese control (OC), the supplemented with aged garlic extract (AGE), the aerobic training (AT), and the supplemented with aged garlic extract + aerobic training (AGE + AT). The rats in the standard diet group also continued their diet for 8 weeks and interventions continued for 8 weeks. AGE was fed to rats once daily at a dose of 600 mg/kg by gavage. AT also included running on a treadmill five days a week. The TLR4 and NFƙB gene expression was measured by Real-time PCR and lipid profile changes evaluated in plasma.
Results: All interventions led to a significant decrease in TLR4 and NFƙB gene expression in visceral fat, as well as improving the lipid profile of obese rats (p = 0.001). TLR4 reduction was higher in AGE + AT group (p = 0.001). On the other hand, AGE and AGE + AT caused a greater decrease in NFƙB gene expression compared to AT (p = 0.001). AGE + AT also improved the lipid profile more compared to other interventions (p = 0.001).
Conclusion: The combining of interventions is more effective in reducing TLR4 and NFƙB gene expression in visceral fat as well as improving lipid profile.
|
|
Keywords: Aerobic training, Obesity, Aged garlic extract, NFƙB, TLR4 |
|
Full-Text [PDF 1136 kb]
(660 Downloads)
|
Type of Study: Original Research |
Subject:
Endocrine
|
|
|
|
|
Add your comments about this article |
|
|