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The effect of ghrelin hormone on level of tumor necrosis factor alpha and gene expression of cytochrome b and interleukin 10 in substantia nigra in an animal model of Parkinson’s disease
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Neda Nikokalam Nazif   , Maryam Khosravi *  , Ramesh Ahmadi , Maryam Bananej , Ahmad Majd |
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Abstract: (2092 Views) |
Background and aims: Inflammatory pathways play an important role in incidence and progression of Parkinson’s disease (PD). Ghrelin has anti-inflammatory and antioxidant effects and inhibits the release of inflammatory cytokines such as tumor necrosis factor alpha (TNF-α) .This study has aimed to investigate the effect of ghrelin hormone on amount of TNF-α, and gene expression of cytochrome b and interleukin10 in Parkinson’s disease induced by1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in male mice.
Methods: Thirty male NMRI mice were randomly divided to 5 groups (six mice in each) including control, saline, ghrelin hormone, Parkinson, and Parkinson + ghrelin hormone. Parkinson’s disease was induced by intraperitoneal (i.p.) injection of 25mg/kg MPTP for 4 days. Ghrelin was injected for 28 days (0.4μg/kg, i.p.). Catalepsy was evaluated by bar test at the 1st, 7th, 14th, 21th, and 28th day after injection of MPTP. TNF-α level and gene expression of cytochrome b, and interleukin10 were measured in Substantia nigra and Corpus striatum by ELISA and Real time PCR techniques at the 29th day.
Results: Catalepsy and TNF-α level significantly increased in substantia nigra and corpus striatum of the Parkinson group, whereas gene expression of cytochrome b, and interleukin10 decreased (p < 0.001). On the other hand, ghrelin hormone significantly decreased catalepsy and TNF-α level while increased gene expression of cytochrome b, and interleukin10 (p < 0.001).
Conclusion: It seems that ghrelin hormone improves catalepsy, decreases TNF-α level, and increases gene expression of cytochrome b, and interleukin10 by inhibiting inflammatory pathways involved in PD.
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| Keywords: Parkinson, Catalepsy, Ghrelin, 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine |
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Full-Text [PDF 1636 kb]
(901 Downloads)
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Type of Study: Original Research |
Subject:
Neuroscience
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