Iranian Journal of Physiology and Pharmacology
مجله فیزیولوژی و فارماکولوژی ایران
Ir J Physiol Pharmacol
Medical Sciences
http://ijpp.phypha.ir
1
admin
2588-4042
2588-4050
8
14
8888
13
fa
jalali
1400
12
1
gregorian
2022
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online
1
fulltext
fa
تعدیل اثرات استرس مزمن از طریق افزایش فعالیت سیستم آدرنرژیک
Modulating the effects of chronic stress through increasing the activity of the adrenergic system
علوم اعصاب
Neuroscience
پژوهشي اصیل
Original Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-family:"B Mitra"">زمینه و هدف:</span></b><span lang="FA" style="font-family:"B Mitra""> <span style="color:black">سیستم نورآدرنرژیک در بروز رفتارهای مرتبط با استرس و اضطراب دخیل است. مسیرهای عصبی استرس و سیستم نورآدرنرژیک در هیپوکمپ اثرگذاری دوطرفه بر یکدیگر دارند. لذا، در این مطالعه به بررسی اثر فعالسازی سیستم نورآدرنرژیک بر اضطراب و یادگیری در شرایط استرس پرداخته می­شود.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-family:"B Mitra"">روش کار:</span></b><span lang="FA" style="font-family:"B Mitra""> <span style="color:black">به موش­های نر بالغ با وزن230-180 نژاد ویستار، استرس مقیدکننده به­صورت حاد (3 روز) یا مزمن (21 روز) داده شد. یوهمبین با دوز 2 میلی­گرم/کیلوگرم (تجویز داخل صفاقی) جهت فعالسازی سیستم نورآدرنرژیک تزریق گردید. پس از 24 ساعت از آخرین جلسه استرس، یادگیری اجتماعی و سپس اضطراب و رفتار جستجوگرانه و تشخیص </span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">شیئ </span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">جدید در حیوانات بررسی گردید.</span></span><span lang="FA" style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-family:"B Mitra"">یافته­ ها:</span></b><span lang="FA" style="font-family:"B Mitra""> میزان همجواری با موش آشنا (غریبه 1) نسبت به کنترل در اثر استرس حاد (0/01 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) و مزمن (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) کاهش و با موش غریبه (غریبه 2) افزایش یافت. زمان حضور در بازوی باز ماز بعلاوه مرتفع در اثر استرس حاد و مزمن و تجویز یوهمبین نسبت به کنترل کاهش (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) و در بازوی بسته (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) افزایش پیدا کرد. میزان بیحرکتی در آزمون زمینه باز در اثر استرس حاد (0/01 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) و مزمن (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) افزایش یافت، درحالی­که بدنبال تجویز یوهمبین در گروه استرس مزمن کاهش (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) یافت. زمان گذرانده شده با </span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">شیئ</span></span><span lang="FA" style="font-family:"B Mitra""> آشنا در اثر استرس مزمن افزایش (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) و در شرایط استرس حاد و تجویز یوهمبین کاهش (۰/001 </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">p </span></span></i><span lang="FA" style="font-family:"B Mitra"">) یافت.</span><span lang="FA" style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">نتیجه­ گیری:</span></span></b><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""> <span style="color:black">این مطالعه نشان داد که افزایش فعالیت سیستم نورآدرنرژیک باعث تعدیل اثرات استرس مزمن می­گردد، که حاکی ازهمکاری احتمالی سیستم گلوکوکورتیکوئیدی و آدرنرژیک می باشد.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:5.0pt"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Background and aims:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""> <span style="color:black">Noradrenergic system is involved in stress and anxiety related behaviors. The neural pathways of stress and the noradrenergic system in the hippocampus have a reciprocal effect on each other. Therefore, in this study, the effect of noradrenergic activity on anxiety and learning under stress conditions is investigated.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Methods:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""> <span style="color:black">Adult male Wistar rats weighing 180-230 were given acute (3 days) or chronic (21 days) restrained stress. Yohimbine was injected with a dose of 2 mg/kg (intraperitoneally) to activate the noradrenergic system. After 24 hours from the last stress session, social learning and then anxiety and exploratory behavior and new object recognition were investigated in animals.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Results:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""> <span style="color:black">The amount of association with a familiar rat (stranger 1) decreased due to acute (<i>p</i> < 0.01) and chronic<br>
(<i>p</i> < 0.001) stress and increased with a stranger rat (stranger 2). The time in the open arm of the elevated plus maze decreased (<i>p</i> < 0.001) due to acute and chronic stress, and animals receiving yohimbine, while the time increased<br>
(<i>p</i> < 0.001) in the closed arm. The duration of immobility in the open field test increased due to acute (<i>p</i> < 0.01) and chronic (<i>p</i> < 0.001) stresses, while it decreased after yohimbine administration in the chronic (<i>p</i> < 0.001) stress group. The time spent with the familiar object was increased (<i>p</i> < 0.001) by chronic stress and decreased (<i>p</i> < 0.001) by acute stress and yohimbine administration.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:120%"><span style="font-family:Verdana,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b> <span style="font-family:"Times New Roman","serif""><span style="color:black">This study showed that increasing the activity of the noradrenergic system moderates the effects of chronic stress, which indicates the possible cooperation of the glucocorticoid and adrenergic systems.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span dir="RTL" lang="FA" style="font-size:5.0pt"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
استرس, سیستم نورآدرنرژیک, یادگیری و حافظه, اضطراب
Stress, Anxiety, Noradrenergic system, Learning and memory
229
239
http://ijpp.phypha.ir/browse.php?a_code=A-10-394-7&slc_lang=fa&sid=1
Masoumeh
Sarfi
معصومه
صرفی
10031947532846007151
10031947532846007151
No
School of biology, Damghan University, Damghan, Iran
دانشکده زیستشناسی، دانشگاه دامغان، دامغان، ایران
Mahmoud
Elahdadi Salmani
محمود
اله دادی سلمانی
10031947532846007152
10031947532846007152
Yes
School of biology, Damghan University, Damghan, Iran
دانشکده زیستشناسی، دانشگاه دامغان، دامغان، ایران
Taghi
Lashkarbolouki
لشکربلوکی
تقی
10031947532846007153
10031947532846007153
No
School of biology, Damghan University, Damghan, Iran
دانشکده زیستشناسی، دانشگاه دامغان، دامغان، ایران
Iran
Goudarzi
ایران
گودرزی
10031947532846007154
10031947532846007154
No
School of biology, Damghan University, Damghan, Iran
دانشکده زیستشناسی، دانشگاه دامغان، دامغان، ایران