Iranian Journal of Physiology and Pharmacology
مجله فیزیولوژی و فارماکولوژی ایران
Ir J Physiol Pharmacol
Medical Sciences
http://ijpp.phypha.ir
1
admin
2588-4042
2588-4050
8
14
8888
13
fa
jalali
1400
12
1
gregorian
2022
3
1
6
online
1
fulltext
fa
پیوند سلولهای بنیادی جنینی موش سوری استرس اکسیداتیو و التهاب را در مدل حیوانی سکته مغزی کاهش می دهد
Transplantation of mouse embryonic stem cell reduces oxidative stress and inflammation in an animal model of stroke
علوم اعصاب
Neuroscience
پژوهشي اصیل
Original Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="tab-stops:right 499.5pt 7.0in"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">زمینه و هدف</span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">:</span></span></span></b><b> </b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">سکته</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مغزی دومین</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">عامل</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مرگ</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">و</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">میر</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">و</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">ناتوانی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">جسمی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">جهان</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">می­باشد.</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">سلول­درمانی به­دلیل توانایی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">ترمیم</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">و</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">جایگزینی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">نورونهای</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">آسیب­دیده</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">می­تواند</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">به­عنوان</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گزینه مناسبی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">نظر گرفته</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">شود. سلولهای بنیادی جنینی موش </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">(mESCs)</span></span></span><span style="line-height:115%"><span style="font-family:"B Mitra""> به­ دلیل آزادسازی فاکتورهای رشد و نروتروفیک</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">عنوان کاندید</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> بالقوه برای تحقیق</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">حاضر</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">نظر</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گرفته</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">شد.</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="tab-stops:right 499.5pt 508.5pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">روشها</span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""> :</span></span></span></b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">موشها به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">سه</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گروه</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">اصلی شاهد، کنترل و</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گیرنده پیوند سلولهای بنیادی جنینی موش </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">(mESCs)</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> تقسیم­ بندی شدند.</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">درگروه </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">mESCs</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">، القای</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">ایسکمی مغزی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">روش انسداد شریان مغزی میانی (</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">MCAO</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">) ،</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">چهار روز پس از تزریق نیم میلیون سلول به استریاتوم مغز موش به روش جراحی استریوتاکسی صورت</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گرفت. موشها به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">زیرگروههایی جهت</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">بررسی</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">نواقص نورولوژیک، حجم انفارکتوس،</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">میزان</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">استحکام</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">سد</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">خونی</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">-</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مغزی،</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">ادم، بررسی فعالیت آنزیم سوپر اکسید دسموتاز </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">(SOD)</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> و بیان ژنهای گیرنده یونوتروپیک گلوتامات </span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">GRIN1</span></span></span></i><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> و نیتریک اکسید سنتاز </span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">NOS1</span></span></span></i><span style="line-height:115%"><span style="font-family:"B Mitra""> تقسیم­ بندی شدند.</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="tab-stops:right 499.5pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">یافتهها:</span></span></b><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گروه </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">mESCs</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">، کاهش</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">معناداری در نواقص نورولوژیک،</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">حجم انفارکتوس،</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">آسیب</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">سد</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">خونی</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">-</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مغزی و</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">ادم</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">نسبت</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گروه</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">کنترل</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مشاهده</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گردید.</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">همچنین بیان ژنهای</span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">GRIN1</span></span></span></i><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">NOS1</span></span></span></i><span style="line-height:115%"><span style="font-family:"B Mitra""> کاهش معناداری را در گروه </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">mESCs</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> نسبت به گروه کنترل نشان داد. همچنین افزایش</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">معنادار بیان آنزیم </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">SOD</span></span></span><span style="line-height:115%"><span style="font-family:"B Mitra""> در</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گروه </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif"">mESCs</span></span></span><span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra""> نسبت</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">به</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گروه</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">کنترل</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">مشاهده</span></span> <span lang="FA" style="line-height:115%"><span style="font-family:"B Mitra"">گردید.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">نتیجه­گیری</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:99%"><span style="font-family:"Times New Roman","serif""> :</span></span></span></b><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">نتایج مطالعه حاضر نشان داده است که<b> </b>پیوند</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:99%"><span style="font-family:"Times New Roman","serif"">mESCs </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""> موجب</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">کاهش آسیبهای ایسکمی</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">گردید</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">که</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">احتمالا بخشی از</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">این</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">اثربه­واسطه</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">کاهش</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">بیان ژنهایدمرتبط با</span></span> <span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra"">التهاب و تحریک­پذیری نورونی و افزایش آنزیم آنتی ­اکسیدانی است.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:5.0pt"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:120%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">Background and Aim</span></span></b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">:</span></span> <span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">Stroke is the second cause of death and disability in the world. Cell therapy can be considered as a suitable option due to its ability to restore and replace damaged neurons. Mouse embryonic stem cells (mESCs) were considered as potential candidates for the present research due to the release of growth and neurotrophic factors.</span></span><span dir="RTL" lang="FA" style="font-size:13.0pt"><span style="line-height:120%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:120%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">Methods:</span></span></b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif""> Rats were divided into three main groups: Intact, control, and mouse embryonic stem cell (mESCs) transplant recipients. In mESCs group, ischemia was induced by middle cerebral artery occlusion (MCAO) method four days after injection of half a million cells into striatum of rat brain by stereotaxic method. Rats were divided into subgroups to investigate neurological deficits, infarct volume, blood-brain barrier integrity, edema, superoxide dismutase (SOD) enzyme activity, and the expression of glutamate ionotropic receptor (GRIN1) and nitric oxide synthase (NOS1) genes. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:120%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">Results</span></span></b><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">:</span></span> <span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">A significant reduction in neurological deficits, infarct volume, blood-brain barrier damage and edema was observed in the mESCs group compared to the control group. Also, the expression of <i>GRIN1</i> and <i>NOS1</i> showed a significant decrease in the mESCs group compared to the control group. In addition, a significant increase in SOD enzyme expression was observed in the mESCs group compared to the control group.</span></span><span dir="RTL" lang="FA" style="font-size:13.0pt"><span style="line-height:120%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:120%"><span style="font-family:Verdana,"sans-serif""><b><span style="font-size:11.0pt"><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">Conclusion</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">: The present results showed that</span></span></span> <span style="font-size:11.0pt"><span style="line-height:120%"><span style="font-family:"Times New Roman","serif"">transplantation of mESCs reduced ischemic damages. This effectiveness is probably due to reduction of inflammatory and excitotoxicity related-genes and increase of antioxidant enzyme.</span></span></span><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:130%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:10.0pt"><span style="line-height:130%"><span style="font-family:"Times New Roman","serif""><span style="color:black">‎</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:130%"><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
ادم, انفارکتوس, سد خونی-مغزی, سلولهای بنیادی جنینی, نقایص نورولوژیک
Edema, Infarct volume, Blood-brain barrier, Embryonic stem cells, Neurological defects
184
200
http://ijpp.phypha.ir/browse.php?a_code=A-10-538-4&slc_lang=fa&sid=1
Mahmoud
Ramdan
محمود
رمدان
10031947532846007133
10031947532846007133
No
1Department of Animal Sciences, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
گروه علوم جانوری، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران
Mohammad Reza
Bigdeli
محمدرضا
بیگدلی
10031947532846007134
10031947532846007134
Yes
1Department of Animal Sciences, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
گروه علوم جانوری، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران
Hosein
Shahsavarani
حسین
شاهسوارانی*
10031947532846007135
10031947532846007135
No
2Deptartment of Cellular and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
گروه زیست شناسی سلولی مولکولی، دانشکده علوم و فناوری زیستی ، دانشگاه شهید بهشتی، تهران، ایران
Salma
Ahmadloo
سلما
احمدلو
10031947532846007136
10031947532846007136
No
3Vaccination Department, Pasteur Institute of Iran, Tehran, Iran
گروه واکسیناسیون، انستیتو پاستور ایران، تهران، ایران