fa بررسی اثر ضدافسردگی عصاره هیدروالکلی زرشک در مدل‌ موشی افسردگی علوم اعصاب Neuroscience پژوهشي اصیل Original Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span b="" mitra="" style="font-family:">زمینه و هدف: </span></span></b><span b="" lang="FA" mitra="" style="font-family:">زرشک دارای اثرات ضددرد، ضدالتهاب، آنتی‌اکسیدان و کاهنده علایم سندرم محرومیت مورفین می‌باشد. با این پیشینه،&nbsp; هدف مطالعه حاضر بررسی اثر ضدافسردگی عصاره هیدرو الکلی زرشک در آزمون شنای اجباری و آزمون معلق ماندن دم می‌باشد.<b><span style="background:white"></span></b></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span b="" mitra="" style="font-family:">روش‌ها<a name="_Hlk160478685">:</a></span></span></b> <span b="" lang="FA" mitra="" style="font-family:">84 سر موش سوری نر&nbsp; آلبینو ( 6 موش در هر گروه)&nbsp; به شکل داخل صفاقی نرمال سالین، فلوکستین (20 میلی‌گرم بر کیلوگرم)، ایمی پرامین&nbsp;</span><span b="" lang="FA" mitra="" style="font-family:">(30 میلی‌گرم بر کیلوگرم) و دوزهای مختلف عصاره هیدروالکلی زرشک (40،20، 80 و 160 میلی‌گرم بر کیلوگرم) را دریافت نموده و سپس تحت آزمون</span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span b="" lang="FA" mitra="" style="font-family:">های شنای اجباری و معلق ماندن دم قرار گرفتند. از آزمون جعبه باز&nbsp; نیز جهت بررسی فعالیت حرکتی موش ها استفاده شد.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span b="" mitra="" style="font-family:">یافته‌ها: </span></span></b><span b="" lang="FA" mitra="" style="font-family:">تمام دوزهای عصاره</span> <span b="" lang="FA" mitra="" style="font-family:">زرشک (به جز دوز</span> <span b="" lang="FA" mitra="" style="font-family:">20 میلی گرم بر کیلوگرم) سبب کاهش مدت زمان بی حرکتی در هر دو آزمون شنای اجباری و معلق ماندن دم می شوند ( 0/001 ></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">p&nbsp;</span></span></i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""> . (</span></span><span b="" lang="FA" mitra="" style="font-family:">علاوه بر این عصاره هیدروالکلی زرشک باعث افزایش مدت زمان شنا کردن( 0/001 ></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">p&nbsp;</span></span></i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp; (</span></span><span b="" lang="FA" mitra="" style="font-family:">بدون تغییر معنی دار در زمان صعود کردن در آزمون شنای اجباری گردید&nbsp;( 0/05</span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">>&nbsp;</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">p&nbsp;</span></span></i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;.(</span></span><span b="" lang="FA" mitra="" style="font-family:">&nbsp;فعالیت حرکتی موش‌ها(تعداد عبور از مربع و روی دوپاایستادن) در آزمون جعبه باز تغییر معنی&shy;داری نشان نداد ( 0/05</span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">>&nbsp;</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">p&nbsp;</span></span></i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""> (</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="FA" style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span b="" mitra="" style="font-family:">نتیجه‌گیری: </span></span></b><span b="" lang="FA" mitra="" style="font-family:">باتوجه&shy;‌به اثرات ضدافسردگی زرشک در هر دو مدل موشی، اثرات ضدافسردگی آن همچون داروهای سروتونرژیک (مثل فلوکستین) می باشد. بااین‌حال، تعیین مکانیسم دقیق اثر آن نیازمند مطالعات بیشتر و گسترده‌تری می باشد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span lang="FA" style="font-size:5.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and Aim</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">:</span></span></span> <i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Berberis vulgaris</span></span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> L. has analgesic, anti-inflammatory, antioxidant, and reducing morphine withdrawal signs effects. With this background, the aim of the present study is to investigate the antidepressant effect of B<i>. vulgaris</i> hydroalcoholic extract (BVHE) in forced swimming test (FST) and tail suspension test (TST). </span></span></span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> <span style="background:white">84 male albino mice (6 mice in each group) were injected intraperitoneally with normal saline, fluoxetine (20 mg/kg), imipramine (30 mg/kg), and different doses of BVHE (40, 20, 80, and 160 mg/kg) and then they were subjected to FST and TST. The open-filed test (OFT) was also used to assess mice locomotion.</span> </span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">:</span></span></span> <span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">All doses of BVHE (except the dose of 20 mg/kg) decrease the duration of immobility in both FST and TST<br> (</span></span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">p</span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> <</span></span></span></span> <span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">0.001). In addition, BVHE increased the duration of swimming (</span></span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">p</span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> < 0.001) without a significant change in the climbing time in FST (</span></span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">p</span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> > 0.05). The locomotion of mice (the number of crossings and rearings) did not show any significant change in the OFT (</span></span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">p</span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> > 0.05). </span></span></span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">: Due to B. <i>vulgaris</i> anti-depressant effects in both mouse models, its antidepressant effects are similar to</span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">those of serotonergic drugs (such as fluoxetine). However, determining the exact mechanism of its action requires more and more extensive studies.</span></span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span><br> &nbsp; آزمون شنای اجباری, استرس اکسیداتیو, زرشک, ضدافسردگی Forced swimming test, Oxidative stress, Berberis vulgaris L., Antidepressant 149 157 http://ijpp.phypha.ir/browse.php?a_code=A-10-631-2&slc_lang=fa&sid=1 Reza Sadri رضا صدری 10031947532846006564 10031947532846006564 No Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران Ghader Najafi قادر نجفی 10031947532846006565 10031947532846006565 Yes Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران Saeid Abbasi-Maleki سعید عباسی ملکی 10031947532846006566 10031947532846006566 No Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran مرکز تحقیقات دارویی، مؤسسه سلامت، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران