fa نقش سیستم هیستامینرژیک مرکزی در کم‌خوری القایی توسط کوله سیستوکینین در جوجه‌های گوشتی علوم اعصاب Neuroscience پژوهشي اصیل Original Research <span style="font-size:11pt"><span style="line-height:98%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span style="line-height:98%"><span b="" mitra="" style="font-family:">زمینه و هدف: </span></span></span></b><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">علیرغم اثبات نقش کوله‌سیستوکینین (</span></span><span dir="LTR" lang="EN-GB" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">) و سیستم هیستامینرژیک مرکزی در تنظیم اخذ غذای پرندگان و پستانداران، تاکنون یافته‌ای با مضمون تداخل اثر میان این دو سیستم ارائه نشده است. از این رو، مطالعه حاضر با هدف بررسی نقش گیرنده‌های هیستامینرژیک مرکزی در تغییرات اخذ غذای ناشی از تجویز </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> در جوجه‌های گوشتی طراحی و اجرا گشت.&nbsp; <b><span style="background:white"></span></b></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:98%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span style="line-height:98%"><span b="" mitra="" style="font-family:">روش‌ها<a name="_Hlk160478685">:</a></span></span></span></b> <span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">به &shy;این&shy; منظور جوجه‌ها در سن پنج روزگی به صورت داخل بطن مغزی (</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">ICV</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">) تزریق شدند. در آزمایش اول محلول کنترل و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> در دوزهای 25/0، 5/0 و 1 نانومول و در آزمایش دوم محلول کنترل،</span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_{8S&nbsp;&nbsp;}</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">(1 نانومول)، </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">&alpha;-FMH</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> (مهارکننده سنتز هیستامین) و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S + &alpha;-FMH</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> تجویز شد. آزمایش‌های 5-3 مشابه آزمایش دوم بودند با این تفاوت که به جای </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">&alpha;-FMH</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">، کلرفنیرامین (آنتاگونیست گیرنده </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">H1</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">)، فاموتیدین (آنتاگونیست گیرنده </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">H2</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">) و تیوپرامید (آنتاگونیست گیرنده </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">H3</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">) تزریق شدند. سپس مصرف تجمعی غذا در فواصل 30، 60 ، 120 دقیقه پس از تزریق اندازه‌گیری شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:98%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span style="line-height:98%"><span b="" mitra="" style="font-family:">یافته‌ها: </span></span></span></b><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">با توجه&shy; به نتایج بدست&shy;آمده، تزریق </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> بطور وابسته به دوز موجب کاهش مصرف غذا در جوجه‌ها شد (0/05</span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">p <&nbsp;</span></span></span></i><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times=""> </span></span></span></i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times=""> </span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:98%"><span b="" mitra="" style="font-family:">.</span></span></span> <span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">همچنین علیرغم عدم اثرگذاری تزریق مستقل دوزهای تحت اثر آنتاگونیست‌های گیرنده‌های هیستامینی، تزریق توامان </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">&alpha;-FMH</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> و کلرفنیرامین با </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> به&shy;طور معنی‌‌داری کاهش اخذ غذای ناشی از </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> را تضعیف نمود (0/05</span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">p <&nbsp;</span></span></span></i><span lang="FA" style="line-height:98%"><span style="font-family:"B Mitra""></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times=""> </span></span></span></i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times=""> </span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:98%"><span b="" mitra="" style="font-family:">.</span></span></span><b><span lang="FA" style="background:white"><span style="line-height:98%"><span style="font-family:"B Mitra""></span></span></span></b></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:98%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="background:white"><span style="line-height:98%"><span b="" mitra="" style="font-family:">نتیجه‌گیری: </span></span></span></b><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:">این یافته‌&shy;ها نشان می&shy;‌دهد که احتمالاً اثرات هیپوفاژیک </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">CCK_8S</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> توسط گیرنده‌های </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:98%"><span new="" roman="" style="font-family:" times="">H1</span></span></span><span lang="FA" style="line-height:98%"><span b="" mitra="" style="font-family:"> در جوجه‌های گوشتی میانجیگری می‌شود.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:98%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span lang="FA" style="font-size:5.0pt"><span style="line-height:98%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and Aim</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">:</span></span></span> <span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Despite proving the role of cholecystokinin (CCK) and the central histaminergic system in the regulation of food intake in birds and mammals, no findings have been presented regarding the interaction between these two systems. Therefore, the present study was designed and implemented with the aim of investigating the role of central histaminergic receptors in changes in food intake caused by CCK8S administration in broiler chickens.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> <span style="background:white">For this purpose, chicks were injected intracerebroventricularly (ICV) at the age of five days. In the first experiment, control solution and CCK8S in doses of 0.25, 0.5, and 1 nmol were administered, and in the second experiment, control solution, CCK8S (1 nmol), &alpha;-FMH (histamine synthesis inhibitor) and CCK8S + &alpha;-FMH were administrated. Experiments 3-5 were similar to the second experiment except that instead of &alpha;-FMH, chlorpheniramine (H1 receptor antagonist), famotidine (H2 receptor antagonist) and thiopramide (H3 receptor antagonist) were injected. Then, cumulative food consumption was measured at 30, 60, and 120 minutes after injection.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">:</span></span></span> <span style="font-size:10.0pt"><span style="background:white"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">According to the obtained results, CCK8S injection decreased food consumption in chickens in a dose-dependent manner (<i>p </i>&le; 0.05). Also, despite the ineffectiveness of separate injection of sub-effective doses of histamine receptor antagonists, the combined injection of &alpha;-FMH and chlorpheniramine with CCK8S significantly weakened the reduction of food intake induced by CCK (<i>p </i>&le; 0.05).</span></span></span></span><b><span style="font-family:"Times New Roman","serif""></span></b></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">: These findings indicate that the hypophagic effects of CCK8S may be mediated via H1 receptors in broilers.</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <br> &nbsp; اخذ غذا, جوجه گوشتی, سیستم هیستامینرژیک, کوله سیستوکینین Food intake, Broiler, Histaminergic system, Cholecystokinin 105 114 http://ijpp.phypha.ir/browse.php?a_code=A-10-115-15&slc_lang=fa&sid=1 Vahid Hamedian Asl وحید حامدیان اصل 10031947532846006465 10031947532846006465 No Department of Basic Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran گروه علوم پایه، دانشکده دامپزشکی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، ایران Morteza Zendehdel مرتضی زنده‌دل 10031947532846006466 10031947532846006466 Yes Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران Keyvan Hasani کیوان حسنی 10031947532846006467 10031947532846006467 No Department of Basic Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran گروه علوم پایه، دانشکده دامپزشکی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، ایران Hamed Zarei حامد زارعی 10031947532846006468 10031947532846006468 No Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران، ایران Kimia Mahdavi کیمیا مهدوی 10031947532846006469 10031947532846006469 No Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران