Iranian Journal of Physiology and Pharmacology
مجله فیزیولوژی و فارماکولوژی ایران
Ir J Physiol Pharmacol
Medical Sciences
http://ijpp.phypha.ir
1
admin
2588-4042
2588-4050
8
14
8888
13
fa
jalali
1403
12
1
gregorian
2025
3
1
8
online
1
fulltext
fa
بررسی اثرات کوتاه مدت و بلند مدت پم-3-سیس برحافظه فضایی موش صحرایی
Investigating the short-term and long-term effects of Pam3cys on the spatial memory of rats
علوم اعصاب
Neuroscience
پژوهشي اصیل
Original Research
<span style="font-size:11pt"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="background:white"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">زمینه و هدف: </span></span></span></span></b><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">پم-3-سیس یک اگونیست ضعیف گیرنده­های شبه تول نوع 1 و 2 است </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">که </span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">قادر است ازطریق مهار ترشح سایتوکاین­های التهابی و افزایش ترشح سایتوکاین­های ضدالتهابی اختلال یادگیری و حافظه در مدل حیوانی بیماری آلزایمر را مهار کند و به ­این ­ترتیب در بهبود عملکرد حافظه موثر باشد. در این مطالعه، اثر داروی پم-3-سیس به تنهایی بر حافظه فضایی موش­های صحرایی سالم بررسی شد.</span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="background:white"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">روشها<a name="_Hlk160478685">:</a></span></span></span></span></b> <span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">این مطالعه بر روی </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">48</span></span></span> <span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">سر موش صحرایی نر بالغ 45 روزه نژاد ویستار با وزن 270-250 گرم انجام شد. موش­ها به­طور تصادفی در</span></span></span> <span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">شش گروه (8 سر </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">در هر گروه)</span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"> شامل دو گروه کنترل 7 و 28 روز، دو گروه شم 7 و 28 روز و دو گروه دارو 7 و 28 روز قرار گرفتند. روند یادگیری حافظه فضایی تمام موش­ها با استفاده از ماز آبی موریس به مدت پنج روز و هر روز چهار جلسه تمرین برای یافتن سکوی پنهان انجام شد. در آخرین روز دوره یادگیری، تزریق از راه داخل بطنی </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">بافر فسفات </span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">(گروه شم) </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">و </span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">یا پم-3-سیس (</span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">1 میکروگرم بر 5 میکرولیتر به ازای هرموش</span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"> ) </span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">انجام شد. 7 و </span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">28 روز پس از تزریق، با اندازه­گیری مدت زمان</span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">، سرعت</span></span></span><span lang="AR-SA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"> و مسافت طی شده در ربع دایره هدف حافظه موش­ها سنجیده شد.</span></span></span><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="background:white"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">یافتهها: </span></span></span></span></b><span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">در مرحله یادگیری، تفاوت معناداری بین </span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">گروه­ها از نظر </span></span><span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">زمان صرف شده و همچنین مسافت طی­شده در ربع دایره هدف و سرعت پیمایش مسافت توسط حیوان ، مشاهده نشد. <i>در</i> زمان­های 7 و 28 روز پس از دوره یادگیری، نیز تفاوت معنی­داری بین</span></span> <span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">گروه­ها از نظر فراخوانی حافظه بین</span></span> <span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">گروه حیواناتی که </span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">پم-3-سیس دریافت نمودند </span></span><span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">با گروه حیوانات کنترل و همچنین شم وجود نداشت (0</span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black">/05</span></span><span dir="LTR" style="font-family:"Times New Roman","serif""><span style="color:black">></span></span> <i><span dir="LTR" style="font-size:8.0pt"><span style="line-height:99%"><span style="font-family:"Times New Roman","serif""><span style="color:black">p</span></span></span></span></i><span lang="AR-SA" style="font-family:"B Mitra""><span style="color:black">).</span></span><span lang="FA" style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-family:"B Mitra""><span style="color:black">نتیجهگیری: </span></span></b><span lang="FA" style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black">پم-3-سیس با دوز استفاده شده در این مطالعه در کوتاه­ مدت و بلندمدت تأثیری بر عملکرد حافظه فضایی موش­های صحرایی ندارد.<b> </b>همچنین به­ علت عدم تأثیر بر موش سالم، می­تواند به­ عنوان داروی مطمئن در شرایط بیماری مورد استفاده قرار گیرد.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:5.0pt"><span style="line-height:99%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Background and Aim</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">:</span></span></span></span> <span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Pam3cys is a weak agonist of toll-like receptors type 1 and 2 and is able to inhibit learning and memory impairment in an animal model of Alzheimer's disease by inhibiting the secretion of inflammatory cytokines and increasing the secretion of anti-inflammatory cytokines. This arrangement is effective in improving memory performance. Considering the improving effect of Pam3cys on learning and memory impairment of Alzheimer's disease in an animal model, in this study, the effect of Pam3cys drug alone on the spatial memory of rats was investigated</span></span></span></span></span><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Methods:</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black"> <span style="background:white">In this experimental study, 48 male Wistar rats (age, 45-days old; weight, 220-270 g) were randomly divided into six groups (n = 8) as follows: There were two control groups of 7 and 28 days, two sham groups of 7 and 28 days, and two drug groups of 7 and 28 days. The spatial memory learning process of all mice was done using the Morris water maze for five days and four training sessions every day to find the hidden platform. At the last day of the learning period, the drug was injected intraventricularly with phosphate buffer (sham group) or Pam3cys (1 μg/5μl per mouse). 7 and 28 days after the injection, the memory of the rats was measured by measuring the time, speed and distance traveled in the target quarter of the maze.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Results</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">:</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">In the learning phase, there was no significant difference between the groups in terms of the time spent and the distance traveled in the target quarter of the circle. At 7 and 28 days after the learning period, there was no significant difference between the groups in terms of memory recall between the group of animals that received Pam3cys and the group of control animals as well as sham animals without receiving the drug (<i>p</i> > 0.05).</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Conclusion</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><span style="color:black">: <span style="background:white">Pam3cys</span> with the dose used in this study has no effect on the spatial memory performance of rats in the short and long term. Also, due to the lack of effect on healthy rats, it can be used as a safe medicine in disease conditions.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></span></span></span></span></span><br>
پم-3-سیس, حافظه فضایی, مازآبی موریس, موش صحرایی
Pam3cys, spatial memory, Morris water maze, Rat
23
31
http://ijpp.phypha.ir/browse.php?a_code=A-10-616-2&slc_lang=fa&sid=1
Mahbobeh
Kamrani Mehni
محبوبه
کامرانی مهنی
10031947532846006299
10031947532846006299
No
Department of Physiology, Faculty of Veterinary Medicine University of Tehran, Tehran, Iran
بخش فیزیولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
Maryam
Hooshmand
مریم
هوشمند
10031947532846006300
10031947532846006300
No
Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
دانشکده علوم، بخش شیمی، دانشگاه فردوسی مشهد، مشهد، ایران
Mohamamd
Sayyah
محمد
سیاح
10031947532846006301
10031947532846006301
Yes
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
بخش فیزیولوژی و فارماکولوژی، انستیتو پاستور ایران، تهران، ایران
Hamid
Gholami Pourbadie*
حمید
غلامی پوربدیع*
10031947532846006302
10031947532846006302
No
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
بخش فیزیولوژی و فارماکولوژی، انستیتو پاستور ایران، تهران، ایران
Morteza
Zendehdel
مرتضی
زنده دل
10031947532846006303
10031947532846006303
No
Department of Physiology, Faculty of Veterinary Medicine University of Tehran, Tehran, Iran
بخش فیزیولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران