fa تأثیر یک دوره تمرین تناوبی با شدت متوسط (MIIT) همراه با مصرف مکمل زنجبیل بر بیان ژن PGC-1α و ATPIF1 در عضله دوقلوی موش‌های صحرایی نر مسن فیزیولوژی ورزش Exercise physiology پژوهشي اصیل Original Research <span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">زمینه و هدف:</span></span></b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> فعال&shy;‌کننده مشترک گیرنده گاما که با تکثیرکننده پراکسی&shy;زوم یک آلفا فعال شده </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PGC-1&alpha;</span></span></span></i><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">) و مهارکننده </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPase</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> فاکتور یک (</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPIF1</span></span></span></i><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">)، در بیوژنز میتوکندری نقش دارند. هدف از پژوهش حاضر تعیین <a name="_Hlk157878745">تأثیر یک دوره تمرین تناوبی </a></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">با شدت متوسط (</span></span><span dir="LTR" lang="X-NONE" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIIT</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">)</span></span> <span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">همراه با مصرف مکمل زنجبیل بر بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PGC-1&alpha;</span></span></span></i><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPIF1</span></span></span></i> <span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">در عضله دوقلوی موش&shy;‌های صحرایی نر مسن بود.</span></span></span></span><br> <span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">روش&shy;‌ها:</span></span></b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> در پژوهشی تجربی، 20 سر موش صحرایی نر مسن نژاد ویستار </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">22 ماهه با وزن 100 </span></span><span lang="FA" style="color:black">&plusmn; </span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">500</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> گرم به طور تصادفی به چهار گروه 5تایی کنترل، تمرین تناوبی، زنجبیل، و تمرین تناوبی + زنجبیل تقسیم شدند. پروتکل تمرینی روی تردمیل به مدت 8 هفته، سه بار در هفته انجام شد. عصاره زنجبیل با دوز 100 میلی&shy;‌گرم به ازای هر کیلوگرم وزن بدن در روز به مدت 8 هفته به صورت خوراکی به موش‌&shy;های صحرایی داده شد. 48 ساعت پس از آخرین جلسه تمرینی برای اندازه&shy;‌گیری ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PGC-1&alpha;</span></span></span></i> <span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPIF1</span></span></span></i><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> از عضله دوقلو بافت‌&shy;برداری شد. جهت مقایسات بین گروهی از آزمون ناپارامتریک کروسکال والیس و جهت مقایسه دو به دوی گروه&shy;ها از آزمون ناپارامتریک یو من ویتنی استفاده شد.</span></span></span></span><br> <span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">یافته&shy;‌ها:</span></span></b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> تنها تفاوت معنی&shy;‌داری در بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PGC-1&alpha;</span></span></span></i> <span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">بین گروه&shy;‌های تمرین تناوبی + زنجبیل و کنترل مشاهده شد </span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">(0/05</span></span></span></span><span style="font-size:8.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">></span></span></span></span><i><span style="font-size:9.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></span></i><span style="font-size:8.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> </span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">).</span></span></span></span><span style="line-height:normal"><span style="tab-stops:right 22.5pt"><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">&nbsp;همچنین، تفاوت معنی&shy;داری در بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPIF1</span></span></span></i><i> </i><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">بین گروه&shy;های پژوهش وجود نداشت</span></span></span></span><span dir="RTL" lang="FA" style=""><span style="line-height: 107%;"><span b="" mitra="" style="font-family: "><span style="color: black;"><span style="font-size: 11pt;">(0/05</span><span style="font-size: 10.6667px;"><</span></span></span></span></span><i><span style="font-size:9.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></span></i><span style="font-size:8.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> </span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span></span><span style="line-height:normal"><span style="tab-stops:right 22.5pt"><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">نتیجه&shy;‌گیری:</span></span></b><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> هشت هفته </span></span><span dir="LTR" lang="X-NONE" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIIT</span></span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> همراه با مصرف مکمل زنجبیل می&shy;‌تواند منجر به افزایش بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PGC-1&alpha;</span></span></span></i> <span b="" lang="FA" mitra="" style="font-family:"><span style="color:black">در عضله دو قلوی موش&shy;‌های صحرایی نر مسن شود، اما باعث تغییر معنی&shy;‌داری در بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATPIF1</span></span></span></i><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"> نشد.</span></span><span b="" lang="FA" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background and Aim: </span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Peroxisome proliferator-activated receptor gamma coactivator 1-alpha</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>PGC-1&alpha;</i>) and ATPase inhibitory factor 1 (<i>ATPIF1</i>) play a role in mitochondrial biogenesis. </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The aim of the present study was to determine the effects of a period of moderate-intensity interval training (MIIT) with ginger supplementation on <i>PGC-1</i></span></span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&alpha;</span></span></span></span></i> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">and <i>ATPIF1</i> gene expression</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> in</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">gastrocnemius muscle of aged male rats.</span></span></span></span><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></b></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Methods: </span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">In an experimental research, 20 aged male Wistar rats (22-month-old with a weight of 500 </span></span></span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&plusmn;</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">100 g)</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">were randomly divided into four groups of 5 each: control, interval training, ginger, and interval training + ginger. Training protocol</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">was conducted on a treadmill for 8 weeks, three times a week. Ginger extract was orally administered to rats at a dose of 100 mg/kg body weight per day for 8 weeks. Forty-eight hours after last training session, tissue removal was performed from gastrocnemius muscle to measure the expression of <i>PGC-1&alpha;</i> and <i>ATPIF1</i> genes. Non-parametric</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Kruskal-Wallis test was used for inter-group comparisons, and non-parametric Mann-Whitney U test was used to compare two groups.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results: </span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Only a significant difference was observed in <i>PGC-1&alpha;</i> gene expression between interval training + ginger and control groups (<i>p</i> < 0.05). Also, there was no</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">significant difference in <i>ATPIF1</i> gene expression between research</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">groups (<i>p</i> > 0.05).</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Eight weeks of </span></span></span></span><span lang="X-NONE" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIIT</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> with ginger supplementation can lead to an increased <i>PGC-1&alpha;</i></span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">gene expression in gastrocnemius muscle of aged male rats, but it did not cause a significant</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">change in <i>ATPIF1</i> gene expression</span></span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></b></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span dir="RTL" lang="FA" style="font-size:5.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br> &nbsp; بیوژنز میتوکندری, تمرین تناوبی, زنجبیل, سالمندی, فعال کننده مشترک گیرنده گاما که با تکثیرکننده پراکسی زوم یک آلفا فعال شده, مهارکننده ATPase فاکتور یک Mitochondrial biogenesis, Interval training, Ginger, Aging, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 240 249 http://ijpp.phypha.ir/browse.php?a_code=A-10-352-8&slc_lang=fa&sid=1 Fatemeh Ramezani Vavdare فاطمه رمضانی واودره 10031947532846006181 10031947532846006181 No 1Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا (س)، تهران، ایران Parvaneh Nazarali پروانه نظرعلی 10031947532846006182 10031947532846006182 No 1Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا (س)، تهران، ایران Abolfazl Shakibaee ابوالفضل شکیبایی 10031947532846006183 10031947532846006183 No 2Exercise Physiology Research Center, Lifestyle Research Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran مرکز تحقیقات فیزیولوژی ورزش، پژوهشکده سبک زندگی، دانشگاه علوم پزشکی بقیه الله (عج)، تهران، ایران Fahimeh Kazemi فهیمه کاظمی 10031947532846006184 10031947532846006184 Yes 1Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا (س)، تهران، ایران