Iranian Journal of Physiology and Pharmacology
مجله فیزیولوژی و فارماکولوژی ایران
Ir J Physiol Pharmacol
Medical Sciences
http://ijpp.phypha.ir
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2023
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تأثیر 8 هفته تمرین تناوبی پر شدت بر استرس شبکه آندوپلاسمی بافت کبد در رتهای چاق مبتلا به دیابت نوع2
Effect of 8 weeks high-intensity interval training on the stress of liver endoplasmic reticulum in obese rats with type 2 diabetes
فیزیولوژی ورزش
Exercise physiology
پژوهشي اصیل
Original Research
<span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span lang="FA" style="font-family:"B Mitra"">زمینه و هدف:</span></b> <span lang="FA" style="font-family:"B Mitra"">تحقیقات اخیر نشان دادهاند که استرس شبکه آندوپلاسمی در بافت کبد نقش بسزایی در پیشرفت و ایجاد دیابت نوع 2 دارد. هدف این پژوهش بررسی تأثیر 8 هفته تمرین تناوبی پرشدت بر استرس شبکه آندوپلاسمی بافت کبد در موشهای مبتلا به دیابت نوع 2 بود</span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">.</span></span><span lang="FA" style="font-family:"B Mitra""></span></span></span><br>
<span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span lang="FA" style="font-family:"B Mitra"">روش­ها:</span></b> <span lang="FA" style="font-family:"B Mitra"">برای ­این ­منظور، 24 موش ­صحرایی نر نژاد ویستار با سن 8 هفته و میانگین وزن تقریبی 250-200 گرم تهیه شدند. گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل سالم</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">" </span></span><span lang="FA" style="font-family:"B Mitra"">(8 موش) به­مدت 13 هفته دسترسی آزادانه به غذای استاندارد و آب آشامیدنی داشتند. 16 موش دیگر بمدت 4 هفته تحت رژیم غذای پرچرب قرار گرفتند. سپس این 16 موش را با استرپتوزوتوسین دیابتی نمودیم. بعد از اطمینان از دیابتی ­شدن، موش­ها در دو گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> (ادامه رژیم پرچرب به ­مدت 9 هفته بدون فعالیت ورزشی)، و </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">ورزش دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> (ادامه رژیم پرچرب، یکهفته آشنایی با دستگاه تردمیل و سپس انجام 8 هفته تمرین تناوبی پرشدت)، قرار داده شدند.</span> <span lang="FA" style="font-family:"B Mitra"">48 ساعت پس از آخرین تمرین، میزان بیان فاکتور آغازگر یوکاریوتی 2-آلفا </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif""> (eIF-2-α) </span></span><span lang="FA" style="font-family:"B Mitra"">و فاکتور نکروزه کننده تومور آلفا </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">(TNF-α) </span></span><span style="font-family:"B Mitra""> در بافت کبد همه موش­ها با روش وسترن بلات اندازه­گیری شد.</span><span lang="FA" style="font-family:"B Mitra""></span></span></span><br>
<span style="line-height:normal"><span style="tab-stops:right 22.5pt"><b><span lang="FA" style="font-family:"B Mitra"">یافته­ها:</span></b> <span lang="FA" style="font-family:"B Mitra"">میزان قند خون و فاکتورهای </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">eIF2- α</span></span><span style="font-family:"B Mitra""> و </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">TNF-α</span></span><span lang="FA" style="font-family:"B Mitra""> در کبد موش­های </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> به­طور معنی­داری بیشتر از گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل سالم</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> بود</span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif""><span style="color:black"> </span></span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Mitra"">(<span style="color:black">0/05 > </span></span></span></span><i><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><span style="color:black">p</span></span></span></span></i><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><span style="color:black"> </span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Mitra""><span style="color:black">)</span></span></span></span><span style="line-height:normal"><span style="tab-stops:right 22.5pt"><span lang="FA" style="font-family:"B Mitra""> در گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">ورزش دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> میزان قند خون بطور فاحش و معنی داری کمتر از گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> بود </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif""><span style="color:black"> </span></span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Mitra"">(<span style="color:black">0/05 > </span></span></span></span><i><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><span style="color:black">p</span></span></span></span></i><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><span style="color:black"> </span></span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Mitra""><span style="color:black">) </span></span></span></span><span style="line-height:normal"><span style="tab-stops:right 22.5pt"><span lang="FA" style="font-family:"B Mitra"">اما هیچکدام از فاکتورهای </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">eIF2- α</span></span><span style="font-family:"B Mitra""> و </span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">TNF-α</span></span><span style="font-family:"B Mitra""> تغییر معنیداری نسبت به گروه </span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra"">کنترل دیابتی</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Times New Roman","serif"">"</span></span><span lang="FA" style="font-family:"B Mitra""> نشان ندادند.</span><span lang="FA" style="font-family:"B Mitra""></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-family:"B Mitra"">نتیجه­گیری­:</span></b> <span lang="FA" style="font-family:"B Mitra"">تمرین تناوبی شدید با مشخصات بکار رفته در این تحقیق نتوانست دو مارکر استرس شبکه آندوپلاسمی بافت کبد در موش­های مبتلا به دیابتی نوع 2 موثر را تغییر دهد.</span><span lang="FA" style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:5.0pt"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Background and Aim: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Recent research has shown that stress of endoplasmic reticulum (ER) in liver tissue plays a significant role in development of type 2 diabetes. The aim of this study was to investigate the effect of 8 weeks high-intensity interval training on two known markers of liver ER stress in rats with type 2 diabetes.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Methods: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Twenty four male Wistar rats (8 weeks age and 200-250 g weight) were used. The "healthy control" group (8 mice) had free access to standard food and drinking water for 13 weeks. Another 16 mice were fed a high-fat diet for 4 weeks. Then these 16 rats were made diabetic with streptozotocin and allocated in two groups: "diabetic control" (maintenance of a high-fat diet for 9 weeks without exercise) and "diabetic exercise" (maintenance of a high-fat diet, one week familiarization with treadmill and then 8 weeks high intensity interval training). Then, the expression levels of eukaryotic initiator factor 2-alpha (eIF-2-α) and tumor necrosis factor alpha (TNF-α) in the liver tissue of all rats were measured by western blot method.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Results: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Blood glucose level and expression of both eIF2-α and </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">TNF-α</span></span></span> <span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">were significantly higher <span style="color:black">in the "diabetic control" than the "healthy control" (P≤0.05). The "diabetic exercise" group showed significantly lower glucose level than the the "diabetic control" group (P≤0.05). However, neither eIF-2-α nor TNF-α showed significant change in "diabetic exercise" compared to the "diabetic control" group.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Conclusion: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Intense intermittent exercise with the parameters used in this research could not change two stress markers of liver ER in type 2 diabetic rats.</span></span></span></span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></b></span></span></span></span><br>
استرس شبکه آندوپلاسمی, تمرین تناوبی با شدت بالا, دیابت, چاقی
endoplasmic reticulum stress, high-intensity interval training, diabetes, obesity
219
228
http://ijpp.phypha.ir/browse.php?a_code=A-10-604-2&slc_lang=fa&sid=1
Hadis
Bayat
حدیث
بیات
10031947532846006148
10031947532846006148
No
Department of physical education and sport science, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات علوم انسانی و اجتماعی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Mandana
Gholami
ماندانا
غلامی
10031947532846006149
10031947532846006149
Yes
Department of Professional Physical Education and Sport Science, Kharazmi University, Tehran, Iran
گروه تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران
Hamid
Rajabi
حمید
رجبی
10031947532846006150
10031947532846006150
No
Department of physical education and sport science, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات علوم انسانی و اجتماعی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Hossein
Abed Natanzi
حسین
عابد نطنزی
10031947532846006151
10031947532846006151
No
Department of physical education and sport science, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات علوم انسانی و اجتماعی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران